Intracellular crosstalk signaling downstream of complement and Toll-like receptor activation in acute systemic inflammation using a human whole blood model
As a molecular biologist, I perform basic research in the field of innate immunity, mainly using anticoagulated human whole blood as a model system for acute systemic inflammation.
I am most interested in intracellular signaling processes and their (crosstalk) regulation downstream of complement and CD14/Toll-like receptor activation, especially by pathogens. Among other techniques, I apply multicolor flow cytometry to study cell type specific responses, like protein and mRNA expression as well as posttranslational modification.
Acute inflammation in blood, either sterile or non-sterile, both induces and is a consequence of the interplay between different cascade systems and cell types. The results of my studies emphasize the need to decipher timeframes and cell-type specificities of individual responses in order to understand the innate immune response as a whole.
In addition to my research, I am responsible for the production of recombinant and monoclonal antibodies, which are used as drugs and tools in our studies and analyses.